سلسله معلومة اليوم 10

معلومة اليوم 12-5-2012

من قبل Yasser Ahmed Taha‏ في تحاليل طبية‏ ·

*** Staphylococcus :

 

1- coagulase test : use to differentiate between staphylococcus aureus ( coagulase positive ) from other staphylococcus and streptococcus ( coagulase negative )

 

2- Manitiol salt agar : use to differentiate between staphylococcus aureus, staphylococcus sapropheticus ( ferment manitol salt give yellow colonies ) and staphylococcus epidermides ( not ferment manitol salt not give yellow colocies )

 

3- Novobiocine disk : use to differentiate between staphylococcus aureus , staphylococcus epidermides ( novobiocine sensitive ) and staphylococcus sapropheticus ( novobiocine resistant )

 

*** Streptococcus :

 

1- catalase test : use to differentiate between staphylococcus ( catalase positive ) and all streptococcus ( catalase negative )

 

2- Bacitracin disk : use to differentiate between Streptococcus pyogenes ( Beta hemolytic groups group A ) ( sensitive to bacitracin ) and streptococcus agalactiae ( Beta hemolytic groups group B ) ( resistant to bacitracin ), streptococcus fecalis ( alpha hemolytic group D ) ( resistant to bacitracin )

 

3- bie aesculine hydrolysis and growing in bile agar : use to differentiate between Streptococcus pyogenes ( Beta hemolytic groups group A ) ( variable aesculin hydrolysis and does not grow on bile agar) , streptococcus agalactiae ( Beta hemolytic groups group B ) ( can’t hydrolyse bie aesculine but can grow in bile agar so it can grow in macconkey agar ) , streptococcus fecalis ( alpha hemolytic group D ) ( hydrolyse bie aesculine and can grow in bile agar so it can grow in Macconkey agar media )

 

4- optochin disk : use to differentiate between streptococcus pneumonia ( optochin sensitive ) and streptococcus viridans ( optochin resistant )

 

5- bile solubility test : use to differentiate between streptococcus pneumonias ( bile solubility positive ) and streptococcus viridans ( bile solubility negative )

 

 

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