الحلقه الثالثه من Bacterial infections in humans

من قبل Ahmed Abdallha‏ في تحاليل طبية

السلام عليكم ورحمه الله وبركاته

 

 

أزيكم اخوانى فى الله

 

يارب تكونوا بخير وفى اتم صحه وعافيه

 

النهارده مش هنتكلم فى مزرعه ولكن هنتكلم فى حاجه هامه جدا عشان تقدر تشتغل مزرعتك صح وطلع نتايج صح وتقدر تنمى الكائن بتاعك فى وسط هو بيحبه ويظهر معاك بكل سهوله ويسر

 

هنوضح النهارده بعض النقاط الهامه

 

فى عالم الميكرو بيولوجى عالم الحقيقه والاثارة والاشتياق

 

النهارده هنتكلم عن

 

Classification of culture media

 

لان كل كائن ليه ظروفه الخاصه بيه فيه كائنات بتحب السمك وكائنات بتحب الحمه والمكرونه

وكانت بتحب الشاى سكر زياده

 

وكائنات عمله رجيم عمله ديت واهم حاجه عندها يكون البيبسى يكون دايت

 

تعالى نشوف علماء الميكرو بيولوجى قسموا الميديا الى كام نوع عشان تقدر تحدد انت هتختار انهى نوع من الميديا تشتغل بيه مزرعتك السعيده

 

بس الاول لازم تصلى على حبيبك وحبيبى طب القلوب صل الله عليه وسلم

عليه افضل الصلاة والسلام

Classification of culture media

 

1-       ordinary media

 

a – Liquid ordinary media

b- Solid ordinary media

 

2-      Enriched media

 

a- Blood agar

b- Chocolate agar

c- Loffers serum medium

d -Lowenstein Jensen medium

e -Dorsets egg medium

 

3-         Selective indicator media

 

a- MacConkeys medium

b- Blood Tellurite medium

c- D.C.A (desoxycholate citrate agar )

d- T. C. B .S

 

4-          Enriched media

 

5-           Sugar media

 

خلاص كده عرفنا تقسيمه الميديا الممكن تزرع عليها مزرعتك السعيده

تعالى بقا معايا نعرف تركيبه كل نوع وايه استخدمات كل نوع

 

بس الاول لما تسمعنى الصلاة على افضل حلق الله سيدنا محمد صل الله عليه وسلم

 

ناخد اول نوع وهو

 

 

1- Ordinary media

================

These may be Liquid or solid

 

a -Liquid ordinary media

================

Broth ( Clear yellowish)

 

This is meat infusion to Which 1% peptone 0.5 % Na Cl are added

Peptone water ( clear colorless ) composed of 1% peptone and 0.5 % NaCl

 

Uses Of peptone Water

As base For Sugar mediae

to test for indole production

 

b-   Solid ordinary media

=================-

Nutrient agar   : Agar is prepared from sea weeds called agar agar in the from of powder Tow to three percent of agar is dissolved in broth by boiling then filtered and left to cool at room temp to solidify

 

==============================================================

ثانيا

 

2- Enriched media

================

 

These media are used mainly to culture nutritionally demanding which can not grow on ordinary media eg

Gonococcus,meningococcus,haemophilus,pneumococcus ….

The most frequentiy used enriched media are

 

 

a – Blood agar

===============

this is prepared by melting nutrient agar at 100 Cthen allowing it to cool at 50- 45 Cthen 5-10%defibrinated sterile sheep or horse blood is added and mixed well than left to cool at room temp

blood agar is also used to test the haemolytic activity of different bacteria on erythrocytes

 

alpha- B – no haemolytic at all

 

b- Chocolate agar

==============

 

this is prepared as blood agar but after the addition of blood the temp is raised to 80 C for 2-5 min it is brown in colour

used particularly for the isolation of haemophilus species and pathogenic Neisseria

 

e– Loffer s serum medium

===================

 

composed of three parts horse serum and one part glucose broth

it is asolid opaque whitish medium useful for the growth of corynebacterium diphtheriae

 

d-     Lowenstein Jensen medium

=======================

 

Composed of

beaten eggs ,malachite green,asparagine and glycerol

The medium is poured in screw capped bottles to prevent dehydration and then sterilized in the same way as lofflers serum

it is used for

=======

isolation of mycobacterium tuberculosis     especially from sputum or stool

 

e – Dorsets egg medium

==================

 

Used for the isolaton of tubercle bacilli when present in non contaminated material such as C S F

 

============================================================

ثالثا

 

3- Selective and indicator media

====================

 

a- MacConkey medium

===============

Composed of

 

1-bile or Na taurrocholate to inhibit the growth of non intestinal microorganisms

2-peptone to support growth

3-  Lactose as sugar tested

4- Neutral red indicator to indicate the fermentation of lactose

5- Agar to obtain solid medium

 

Uses

====

 

To differentiate between lactose fermenting and lactose non fermenting enteorbacteriae

 

Lactose fermenterg give rose pink colonies while lactose non fermenters give pale yellow colonies

 

b -Blood tellurite medium

it is used for isolation of corynebacterium diphteriae when it is present in contaminated material

Tellurite inhibits the growth of other bacteria but not that of C diphteriiae

it is also used in classification of C . diphtheriae into mitis ( black colonies ) gravis ( gray colonies ) and intermedius types

 

b – D. C. A (desoxycholate citrate agar )

========================

 

This is used to isolate salmonellae and shigellae Both give pale yellow colonies as they are lactose non fermenters

This medium is selective for salmonellae and shigellae because

 

It inhibit the growth of most non pathogenic intestinal bacteria such as E coli

 

d.= T.C.B.S

===========

 

This is a selective medium used for the isolation of Vibrio cholerae

It is an alkaline agar medium containing sucrose and an indicator dye the commensal intestinal organisms are mostely suppressed and V cholerae grows producing ayellow colony

 

e- Blood tellurite medium

================

it is used for the isolation of Corynebacterium diphteriae when it is present in contaminated material

Telluriae inhibits the growth of other bacteria but not that of C diphtheriae into mitis ( black colonies ) graves ( gray colonies ) and intermedius types

===========================================================

ررابعا

 

4- Enrichment media

==============

these are fluid media , e g selente and tetrathionate broth which contain astimulate the growth of certain pathogenic bacteria as salmonellae and shigellae but not others

 

======================================================

خامسا

 

5-   Sugar media

===========

 

These are media used to test biochemical activity of bacteria on carbohydrates

Composed of sugar , peptone water and Andrade s indicator

A small inverted tube called

Durham s tube

is immersed in the medium for the collection of gases

Litmus milk is a similar medium used to test the action of bacteria on milk composed of free cream milk and few drops of litmus solution

وفى النهايه دى بعض الروابط الهامه لشرح الفيديوا

مقدمة فى طرق زراعة الميكروبيولوجيا    

Introduction to Microbiology Culture Techniques

 

http://www.youtube.com/watch?v=Et1v8EQP10U

 

كيفة تحضير ميديا اجار الدم لزراعة البكتريا

How to make blood agar plate

 

http://www.youtube.com/watch?v=X5dU98-K5_g&feature=related

 

 تحضير ميديا اجار الدم اتوماتيكيا لزراعة البكتريا

Automated making blood agar plates

 

http://www.youtube.com/watch?v=3ZQ9zphKX-s

مقدمة فى ميديا زراعة الميكروبيولوجيا وانواع الميديات المختلفة

Introduction to Microbiology Culture  media

 

http://www.youtube.com/watch?v=EoQEgVxxDxI&feature=related

 

الميديا الاختيارية والميديا التفريقية لزراعة البكتريا

selective and differential media

 

http://www.youtube.com/watch?v=gKA_VLC4-jQ&feature=related

 

 

وجزاكم الله خيرا

اتقدم بالشكر لكل اعضاء الجروب

انتظرونى مع حلقه اخرى

وكائنات اخرى

ومعلومه تهمك فى عالم الميكروبيولوجى

د/احــمــد عبدالله

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