الفرق وطريقه قياس كلا من total ,free , esterified cholesterol

الجــــــــــــــــــــواب:-

 ———————–

 

dr.Ahmed Khaled

………….laboratory assays

Blood samples were collected after an overnight fast into

EDTA-containing Vacutainer tubes on the day of the

angiogram, kept at 4°C, and centrifuged within 12 h.

Aliquots of plasma were frozen at 70°C until analyses.

Plasma total cholesterol (TC), TGs, total HDL-C, and

HDL-unesterified cholesterol (HDL-UC) were measured

enzymatically. LDL-C was calculated using the Friede-

wald equation, and apoB was measured nephelometri-

cally(BeckmanArraySystem).TheradioassayforFERHDL

had been described previously (17–19). Briefly, apoB-

containing lipoproteins are precipitated from EDTA

plasma by phosphotungstic acid and MgCl2. To the su-

pernatant, which contains plasma with HDL only, is 3

added a filter-paper disk containing a trace of [ H]choles-

terol. After an overnight incubation at 4°C, the disk is

removed and the plasma with labeled HDL is heated to

37°C and incubated for 30 min. After the incubation,

lipidsareextractedbyethanolandseparatedbythin-layer

chromatography. FERHDL (%/h) is calculated from the

ratio of radioactive unesterified to radioactive esterified cholesterol.

 

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الســــــــــــــــــــــؤال:-

 ———————–

‏‎Dr.Eman Tawfik‎‏

السلام عليكم 

ولما بيكون عالي ده معناه ايه اصل في حاله طالع معاها اكتر من 600 والطبيعي up to 35 مع العلم انها بتتعالج من ارتفاع TSH من فتره

حد يعرف حاجه عن التحليل thyroid anti -peroxidase Ab

 

الجــــــــــــــــــــواب:-

 ———————–

dr. Ahmed Khaled

 

What does the test result mean?

Negative test results means that the autoantibodies are not present in the blood at the time of testing and may indicate that symptoms are due to a cause other than autoimmune. However, a certain percentage of people who have autoimmune thyroid disease do not have autoantibodies. If it is suspected that the autoantibodies may develop over time, as may happen with some autoimmune disorders, then repeat testing may be done at a later date.

 

Mild to moderately elevated levels of thyroid antibodies may be found in a variety of thyroid and autoimmune disorders, such as thyroid cancer, Type 1 diabetes, rheumatoid arthritis, pernicious anemia, and autoimmune collagen vascular diseases. Significantly increased concentrations most frequently indicate thyroid autoimmune diseases such as Hashimoto thyroiditis and Graves disease.

In general, the presence of thyroid antibodies suggests that there is autoimmune thyroid involvement and the higher the level, the more likely that is. Rising levels may be more significant than stable levels as they indicate an increase in autoimmune activity. All of these antibodies, if present in a pregnant woman, can increase the risk of hypothyroidism or hyperthyroidism in the developing baby or newborn.

If thyroglobulin antibodies are present when the doctor is monitoring thyroglobulin levels, they will interfere with the test results. This may mean that the doctor will not be able to use thyroglobulin as a tumor marker for thyroid cancer. If thyroglobulins antibodies are being used as a monitoring tool and have stayed high or dropped low initially but are increasing over time, then it is likely that the treatment has not been effective and the condition is continuing or recurring. If levels are falling and/or have fallen to low or undetectable levels, then it is more likely that the therapy has been effective.

A certain percentage of people who are healthy may be positive for one or more thyroid antibodies. The prevalence of these antibodies tends to be higher in women, tends to increase with age, and indicates an increased risk of developing thyroid disease in the future. If an individual with no apparent thyroid dysfunction has a thyroid antibody, the doctor will track her health over time. While most may never experience thyroid dysfunction, a few may develop it.

 

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